Multiplex Snp Analysis Microdevice Using Allele-specific Polymerase Chain Reaction-microarray

In this study, we developed an allele-specific polymerase chain reaction-microarray (AS PCR-microarray) chip to perform the allele-specific gene amplification and microarray-based detection on a single wafer to identify Korean indigenous beef cattle, called Hanwoo. For allele-specific discrimination and amplification, Hanwoo-specific ten homozygous single nucleotide polymorphism (SNP) sites were chosen and analyzed by AS PCR on a chip, and the resultant amplicons were verified by the downstream microarray assay. We could identify Hanwoo with 99.3% reliability by detecting the fluorescence signals of the microarray less than three among eleven spots.